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Armed using this information and facts, It will be most informative to trace how archaeal enzymes from intently connected taxa that have really related primary structures arrived to differ in their nucleotide specificities, culminating by introducing amino acids modifications that elicit a acquire of functionality, converting an ATP-only DNA ligase right into a dual-specificity spinoff.

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The movement entails a rigid physique rotation with regard to the hinge phase connecting the NTase and OB domains (indicated with the arrow

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